The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells

The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells

Blog Article

Interleukin-12 (IL-12), produced by dendritic cells in response to activation, is central click here to pathogen eradication and tumor rejection.The trafficking pathways controlling spatial distribution and intracellular transport of IL-12 vesicles to the cell surface are still unknown.Here, we show that intracellular IL-12 localizes in late endocytic vesicles marked by the SNARE VAMP7.Dendritic cells (DCs) from VAMP7-deficient mice are partially impaired in the multidirectional release of IL-12.

Upon encounter with antigen-specific T cells, IL-12-containing vesicles nightstick twm-850xl rapidly redistribute at the immune synapse and release IL-12 in a process entirely dependent on VAMP7 expression.Consistently, acquisition of effector functions is reduced in T cells stimulated by VAMP7-null DCs.These results provide insights into IL-12 intracellular trafficking pathways and show that VAMP7-mediated release of IL-12 at the immune synapse is a mechanism to transmit innate signals to T cells.